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Briefly, explants were cultured in poly-d-lysine (Sigma Aldrich Chemie GmbH, Steinheim, Germany) coated 8-well Ibidi -slides chambers (Vitaris AG, Baar, Switzerland) with 300 l DMEM/ F12 growth medium (Thermo Fisher Scientific, Reinach, Switzerland), 1 B-27 supplement (Thermo Fisher Scientific), 1 N-2 supplement (Thermo Fisher Scientific) and penicillin (30 U/ml, Sigma Aldrich Chemie GmbH) at 37 C and 5% CO 2 for at least 2 h