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Food protein-induced enterocolitis syndrome typically presents in the first 612 months of life with acute symptoms of severe emesis, diarrhea and dehydration that usually occur within 16 h after ingestion of the culprit food

Wilderman A, Guo Y, Taylor S, et al

The bacterial cells were grown at 20 C and after 4048 h, the cells were harvested by the centrifugation at 6500 g for 10 min, washed 23 times with phosphate-buffered saline (PBS) buffer (pH 7.2) and the cell pellet was re-suspended in ice cold 20 mM Tris-Cl (pH 7.4)

On the one hand, tryptophan depletion caused by high expression of indoleamine-2,3-dioxygenase-1 (IDO1) and tryptophan-2,3-dioxygenase (TDO2) in LN229 glioblastoma cells activated the general control non-derepressible-2 (GCN2) kinase, resulting in phosphorylation of eukaryotic translation initiation factor 2 (eIF2) and the activation of activating transcription factor 4 (ATF4), which further upregulated WAS expression