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Principles and Practice of Clinical Parasitology (John Wiley & Sons, Ltd, 2001)

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After blocking with 3% (w/v) milk in PBST for 13 h at room temperature, the plates were washed four times with PBS buffer and incubated with serially diluted purified IgG samples or soluble fractions of crude cell lysates for 1 h at room temperature

T-brachyury is mainly restricted to the notochord and tailbud and has been shown to affect the development of the tail and vertebrae (Wilkinson et al., 1990