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After 2 freeze-thaw cycles, 5% 5-sulfosalicylic acid (SSA, 20 L) was added, and the samples were centrifuged at 8000 g for 10 minutes at 4C

Imaging the substantia nigra in Parkinson disease and other Parkinsonian syndromes

[333] found in the brains of Fmr1 -knockout (KO) mice elevated levels of ROS, GSH, markers of protein oxidation and lipid peroxidation in whole brains, and increased production of NADPH oxidase in the prefrontal cortex, cerebellum, and hippocampus

Other proofs that NAC may have high clinical utility in NUD were provided in reports showing the drug reducing efficacy in models assessing nicotine seeking and reinstatement behaviors [35, 38,39,40,41,42] (Table 1)