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Reaction was stopped by adding 2N H 2 SO 4 and absorbance was recorded at 450 nm

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However, its prolonged use is associated with a major and challenging side effectmorphine tolerance ( Traditionally, research on the mechanisms of morphine tolerance has primarily focused on neurons, particularly involving -opioid receptor (MOR) desensitization, internalization dysfunction, and compensatory upregulation of intracellular cyclic adenosine monophosphate (cAMP) signaling and other adaptive neuronal changes (Zhang et al., 2023

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