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KK1 cells with siRNA transfection were cultured in 96-well plates for 48 h at 37 C in an incubator with 5% CO 2

Also, groups treated with chitosan-coated NLCs (G5 and G6) showed enhanced reduction than corresponding groups treated by crude drugs only (G3 and G4, respectively)

PMID: 28486044

One major concern is that CHAC1 plays a critical role in regulating oxidative stress, GSHdegradation, and ferroptosis, essential processes for cellular redox homeostasis