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Plasmid construction and cell transfection The full-length cDNA encoding human HDAC8 (NM_018486.3) was amplified by PCR and cloned into the pcDNA3.1(+) mammalian expression vector (Invitrogen, Carlsbad, CA, USA) between the HindIII and EcoRI restriction sites to generate the HDAC8 overexpression (HDAC8-OE) plasmid (pcDNA3.1-HDAC8)