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When we correlated VEGF mRNA expression levels with the cyclic changes of perifollicular vascularization, we found that modulations of VEGF expression preceded vascular changes by an interval of approximately 3 days with a marked decrease of VEGF expression during late anagen, catagen, and telogen (Figure 2b)

Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method

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