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Trends in Immunology

ab7291 a mouse anti-tubulin antibody (1/1000) was used to stain tubulin along with ab150120 (Alexa Fluor 594 goat anti-mouse 1/1000) shown in the top right hand panel

Elution is usually complete after 35ml of buffer is collected per 1.0ml of settled resin, provided the imidazole concentration is high enough to efficiently elute the protein of interest