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Tocris), TGF (100 ng ml 1 ) and TRULI (Axon) was added to our previously published mouse hepatoblast medium (MM 29 ) with minor modifications: AdDMEM/F12 (Invitrogen) supplemented with 1% HEPES, 1% GlutaMAX, 1% penicillin/streptomycin, 1 B27 without retinoic acid, 1.25 mM N -acetylcysteine, 10 nM gastrin, 50 ng ml 1 hEGF, 15% RSPO1 conditioned medium, 100 ng ml 1 FGF10, 100 ng ml 1 FGF7, 50 ng ml 1 HGF, 10 mM nicotinamide, 2 M A83-01, 3 M CHIR99021, 10 M Y-27632 and 0.5 nM Wnt surrogate Fc fusion protein

Sikiric P, Sever M, Krezic I, Vranes H, Kalogjera L, Smoday IM, Vukovic V, Oroz K, Coric L, Skoro M, Kavelj I, Zubcic S, Sikiric S, Beketic Oreskovic L, Oreskovic I, Blagaic V, Brcic K, Strbe S, Staresinic M, Boban Blagaic A, Skrtic A, Seiwerth S

A., Ono, K

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