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Then, UPLC-grade water DDW:MetOH (3:1, v/v) solution (0.5 mL) containing internal standards: 13 C- and 15 N-labeled amino acids standard mix (Sigma-Aldrich) was added to the tubes, followed by centrifugation

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doi: 10.1002/jat.1275 75 GaoWChanJY-WWongT-S

For example, a safety study on green tea polyphenols in middle-aged ovariectomized rats showed that daily administration at levels up to 1.5% (w/w) in drinking water (approximately 633 mg/kg body weight/day) for six months was safe without signs of toxicity 65