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Description

Sylvestre, J

The metabolism of these stem-like T cells is poorly understood

The pellet was washed thrice in PBS buffer, resuspended in 10 mM Tris-HCl pH 8.0, 1 mM EDTA pH 8.0, 100 mM NaCl, 0.5% Nonidet P-40, and centrifuged at 16,000 g for 30 min