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The safest reconstitution method involves: (1) Using sterile technique (clean workspace, alcohol swabs, sterile syringes), (2) Using bacteriostatic water for injectionnever tap water, distilled water, or saline, (3) Allowing peptide vials to equilibrate to room temperature before reconstituting, (4) Injecting bacteriostatic water slowly down the vial wall rather than directly onto the powder, (5) Avoiding shaking or vigorous agitation, and (6) Storing reconstituted peptides refrigerated (2-8C) and protected from light

If none of your protein, or very little, is in the flow-through during your purification, then youve used sufficient glutathione agarose (Figure 5, left)

(2022) creates a specific problem for dosing frequency

Oxygen consumption was measured in the basal state, after treatment with oligomycin (2 mg-L-1), a Complex V inhibitor used to determine the relative coupling of respiration and phosphorylation, and FCCP (5 ), a ionophore and mitochondrial uncoupling agent used to determine maximal respiratory capacity of the cells