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Despite these advantages, peptide-based therapies face significant limitations

By analyzing the aortic tissue, we found that FOXO4-DRI treatment significantly increased the expression levels of Ki-67 and Lamin B, while reducing the expression of P21, P16, and -H2AX, similar to the results obtained from naturally aged mice (Figure 2A)

however, VEGF, IL-1a, IL-6, IL-8, MMPs, and chemokines (CXCL2, CXCL3) are secreted by most senescent cells 10,12

( E and F ) Immunoblots of cardiac acetylatedPPAR coactivator 1- (ac-PGC1) ( E ), IgG heavy chain, total PGC1, sirtuin 1 (SIRT1), and -ACTIN ( F ) of C57BL/6 mice fed on regular chow or chow containing tesaglitazar (0.5 mol/kg bw) or a combination of tesaglitazar (0.5 mol/kg bw) and RSV (100 mg/kg bw / day) for 6 weeks (densitometric analysis is shown in Supplemental Figure 5, A and B