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Electrons leak from complex I and III of the electron transport chain (ETC) located on the inner membrane of mitochondria (Zhao et al., 2019)

While aminoglycosides have been shown to perturb membranes through non-specific ionic bonds and misfolded proteins 26,27,28 , another possibility that may explain our observations with both aminoglycosides and fluoroquinolones is that promiscuously reactive metabolic by-products damage membrane phospholipids through processes involving, for example, free-radical reactions 2,3,13,29