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To determine whether NRF2 can localize to the Ptgir gene, we first analyzed NRF2 chromatin immunoprecipitation sequencing (ChIPseq) data from Keap1 / mouse embryonic fibroblasts (MEFs) that display constitutive NRF2 activity 31

Alvarez-Suarez JM, Giampieri F, Tulipani S, Casoli T, Di Stefano G, Gonzlez-Params AM, Santos-Buelga C, Busco F, Quiles JL, Cordero MD, Bompadre S, Mezzetti B, Battino M (2014) One-month strawberry-rich anthocyanin supplementation ameliorates cardiovascular risk, oxidative stress markers and platelet activation in humans

Transwell chambers were assayed for cell invasion ability by applying 100 L of DMEM-diluted Matrigel gel (3:7 dilution) to the chambers to be checked and incubated at 37 C for 30 min to form a gel, then pre-treated MIA PaCa-2 and PANC-1 cells were resuspended with DMEM and spread in small chambers at 50000/well, and the lower chamber was placed in 20% FBS DMEM, incubated at 37C 5% CO 2 for 48 hours

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