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One of the symptoms may be darkening of the skin, especially in body creases

It would certainly be preferable that GSHee be delivered in a dose form that would not elicit profound responses prior to administration of an opioid and we are establishing whether lower doses or a constant infusion can achieve blockade of fentanyl-induced OIRD without eliciting dramatic effects on ventilation prior to administration of the opioid

If the answer is no, consider a slight increase in dose (titration) or a change in timing before abandoning the compound

NHS: Sulfo-NHS-Biotin is water-soluble and ideal for aqueous reactions, while NHS-Biotin requires organic solvent dissolution first