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The amino group of the cysteine residue is then acetylated by the intracellular N-acetyltransferase enzymes and thereby forms the mercapturic acid which, depending on its characteristics, is rapidly led to circulation, bile, urine or metabolized until it is eliminated (Figure 3) [22]

Using a specialized enema kit, the coffee is poured into a bucket (or bag), the nozzle is lubricated and inserted into the rectum, and the coffee is allowed to flow into the colon

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