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To determine whether HDAC9 is necessary for synaptic plasticity in the hippocampus, extracellular recordings of field excitatory postsynaptic potentials (fEPSPs) were conducted in the CA1 area of hippocampal slices from HDAC9 / mice and their WT littermate controls, specifically targeting synapses in the stratum radiatum, where Schaffer collateral inputs from CA3 neurons terminate on the apical dendrites of CA1 pyramidal neurons (Fig