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At predetermined time points (2, 6, 12, and 24 h post-administration), the mice were euthanized, and the residual gel on the skin surface at the application site was removed by washing with PBS

Specifically, enzymes critical for organic matter decomposition and nutrient release such as urease, phosphatase, and dehydrogenase show decreased activity upon ENM exposure, threatening soil fertility (Figure 2) (Luche et al., 2016

It takes getting into the guts of the situation and digging around and testing to discover the actual problem

however, the results have not been as amazing as i had hoped, although i do see some improvements in my skin